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Is it possible to include a 3rd fastq file including only the UMIs, for the FastqToBam step? What I normally do is:
fgbio --compression 1 --async-io FastqToBam --input {input.fqR1} {input.fqR2} {input.umifq} --read-structures +T +T +M --sample {params.samplename} --library {params.samplename} --output {output}
Many thanks, @nh13! Very exciting seeing a nf-core pipeline for fgbio.
The text was updated successfully, but these errors were encountered:
See #11 !
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One thing we need to do is add an example UMI FASTQ file to https://github.com/nf-core/test-datasets for testing purposes.
It would be great to support any subset of the standard four reads that can be generated in a paired end library:
The UMI could be in any of these reads, and FastqToBam would be able to handle it.
See #38
Done!
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Description of feature
Is it possible to include a 3rd fastq file including only the UMIs, for the FastqToBam step? What I normally do is:
fgbio --compression 1 --async-io FastqToBam --input {input.fqR1} {input.fqR2} {input.umifq} --read-structures +T +T +M --sample {params.samplename} --library {params.samplename} --output {output}
Many thanks, @nh13! Very exciting seeing a nf-core pipeline for fgbio.
The text was updated successfully, but these errors were encountered: