diff --git a/Makefile.pre.in b/Makefile.pre.in
index b7b16ef4cb9d19..b74aa8f3003bf6 100644
--- a/Makefile.pre.in
+++ b/Makefile.pre.in
@@ -2249,7 +2249,7 @@ Python/frozen.o: $(FROZEN_FILES_OUT)
 # an include guard, so we can't use a pipeline to transform its output.
 Include/pydtrace_probes.h: $(srcdir)/Include/pydtrace.d
 	$(MKDIR_P) Include
-	CC="$(CC)" CFLAGS="$(CFLAGS)" $(DTRACE) $(DFLAGS) -o $@ -h -s $<
+	CC="$(CC)" CFLAGS="$(CFLAGS)" $(DTRACE) $(DFLAGS) -o $@ -h -s $(srcdir)/Include/pydtrace.d
 	: sed in-place edit with POSIX-only tools
 	sed 's/PYTHON_/PyDTrace_/' $@ > $@.tmp
 	mv $@.tmp $@
@@ -2259,7 +2259,7 @@ Python/gc.o: $(srcdir)/Include/pydtrace.h
 Python/import.o: $(srcdir)/Include/pydtrace.h
 
 Python/pydtrace.o: $(srcdir)/Include/pydtrace.d $(DTRACE_DEPS)
-	CC="$(CC)" CFLAGS="$(CFLAGS)" $(DTRACE) $(DFLAGS) -o $@ -G -s $< $(DTRACE_DEPS)
+	CC="$(CC)" CFLAGS="$(CFLAGS)" $(DTRACE) $(DFLAGS) -o $@ -G -s $(srcdir)/Include/pydtrace.d $(DTRACE_DEPS)
 
 Objects/typeobject.o: Objects/typeslots.inc
 
diff --git a/configure b/configure
index 0e7aefed5ee62d..a4174171ec3555 100755
--- a/configure
+++ b/configure
@@ -19039,15 +19039,27 @@ printf "%s\n" "#define WITH_DTRACE 1" >>confdefs.h
     # linked into the binary. Correspondingly, dtrace(1) is missing the ELF
     # generation flag '-G'. We check for presence of this flag, rather than
     # hardcoding support by OS, in the interest of robustness.
+    #
+    # NetBSD DTrace requires the -x nolibs flag to avoid system library conflicts
+    # and uses header generation for testing instead of object generation.
     { printf "%s\n" "$as_me:${as_lineno-$LINENO}: checking whether DTrace probes require linking" >&5
 printf %s "checking whether DTrace probes require linking... " >&6; }
 if test ${ac_cv_dtrace_link+y}
 then :
   printf %s "(cached) " >&6
 else case e in #(
-  e)             ac_cv_dtrace_link=no
+  e)
+            ac_cv_dtrace_link=no
             echo 'BEGIN{}' > conftest.d
-            "$DTRACE" $DFLAGS -G -s conftest.d -o conftest.o > /dev/null 2>&1 && \
+            case $host in
+                *netbsd*)
+                    DTRACE_TEST_FLAGS="-x nolibs -h"
+                    ;;
+                *)
+                    DTRACE_TEST_FLAGS="-G"
+                    ;;
+            esac
+            "$DTRACE" $DFLAGS $DTRACE_TEST_FLAGS -s conftest.d -o conftest.o > /dev/null 2>&1 && \
                 ac_cv_dtrace_link=yes
        ;;
 esac
@@ -19057,6 +19069,12 @@ printf "%s\n" "$ac_cv_dtrace_link" >&6; }
     if test "$ac_cv_dtrace_link" = "yes"; then
         DTRACE_OBJS="Python/pydtrace.o"
     fi
+    # Set NetBSD-specific DTrace flags in DFLAGS
+    case $host in
+        *netbsd*)
+            DFLAGS="$DFLAGS -x nolibs"
+            ;;
+    esac
 fi
 
 PLATFORM_HEADERS=
diff --git a/configure.ac b/configure.ac
index 1e590e1d0fd727..ee3357bea90e95 100644
--- a/configure.ac
+++ b/configure.ac
@@ -5104,16 +5104,33 @@ then
     # linked into the binary. Correspondingly, dtrace(1) is missing the ELF
     # generation flag '-G'. We check for presence of this flag, rather than
     # hardcoding support by OS, in the interest of robustness.
+    #
+    # NetBSD DTrace requires the -x nolibs flag to avoid system library conflicts
+    # and uses header generation for testing instead of object generation.
     AC_CACHE_CHECK([whether DTrace probes require linking],
-        [ac_cv_dtrace_link], [dnl
+        [ac_cv_dtrace_link], [
             ac_cv_dtrace_link=no
             echo 'BEGIN{}' > conftest.d
-            "$DTRACE" $DFLAGS -G -s conftest.d -o conftest.o > /dev/null 2>&1 && \
+            case $host in
+                *netbsd*)
+                    DTRACE_TEST_FLAGS="-x nolibs -h"
+                    ;;
+                *)
+                    DTRACE_TEST_FLAGS="-G"
+                    ;;
+            esac
+            "$DTRACE" $DFLAGS $DTRACE_TEST_FLAGS -s conftest.d -o conftest.o > /dev/null 2>&1 && \
                 ac_cv_dtrace_link=yes
       ])
     if test "$ac_cv_dtrace_link" = "yes"; then
         DTRACE_OBJS="Python/pydtrace.o"
     fi
+    # Set NetBSD-specific DTrace flags in DFLAGS
+    case $host in
+        *netbsd*)
+            DFLAGS="$DFLAGS -x nolibs"
+            ;;
+    esac
 fi
 
 dnl Platform-specific C and header files.