Biological Oceanography Group (BOG) Protocol Collection Member of Marine Biodiversity Observation Network (MBON) - CeNCOOS MBON Website describing protocols: https://mbari-bog.github.io/MBON-Protocols/ BeBOP formatted detailed protocols with DOIs below: Authors Jacoby Baker Kobun Truelove Katie Pitz Francisco Chavez Protocols Currently in Use Sample Collection Protocols Extraction Protocols Environmental DNA (eDNA) extraction using Qiagen DNeasy 96 Blood and Tissue Kit V.3 PCR Protocols Environmental DNA (eDNA) 18S metabarcoding PCR Protocol V.3 Environmental DNA (eDNA) COI metabarcoding PCR Protocol V.2 Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (with Platinum SuperFi II Taq) V.3 Secondary PCR, Size selection, Library Prep and Sequencing Protocols Protocols Tested PCR Protocols Previously Used Protocols Sample Collection Protocols Extraction Protocols PCR Protocols Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (Touchdown) V.2 Environmental DNA (eDNA) 12S Metabarcoding PCR Protocol (Classic) V.1 Library Prep and Sequencing Protocols